分子别名(Synonym):HCNTF
种属(Species):Human
表达系统(Expressed Host):E. coli
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):The recombinant human CNTF consisting of 214 amino acids and has a calculated molecular mass of 24.5 kDa. The apparent molecular mass of the protein is approximately 27-30 kDa in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):1. Measured by its binding ability in a functional ELISA. Immobilized human His-CNTF (cat:11841-H07E) at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His (Cat:R1243-802B). The EC50 of biotinylated rat CNTFR-His (Cat:R1243-802B) is 60-140 ng/ml.
表达区间(Protein Construction):A DNA sequence encoding the human CNTF (NP_000605.1) (Ala 2-Met 200) was expressed, with a polyhistide tag at the N-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti