分子别名(Synonym):IL10
种属(Species):Sus scrofa (Pig)
表达系统(Expressed Host):E. coli
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4.
分子量(Molecule Mass):The recombinant porcine IL10 consists of 158 amino acids and has a predicted molecular mass of 18.2 kDa. The apparent molecular mass of it is approximately 18 kDa in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):1. Immobilized porcineIL10 at 10 μg/ml (100 μl/well) can bind Cynomolgus IL10RA-Fc (Cat:C1349-202H), The EC50 of Cynomolgus IL10RA-Fc (Cat:C1349-202H) is 0.14-0.34 μg/ml.
表达区间(Protein Construction):A DNA sequence encoding the porcine IL10 (Q29055) (Ser19-Asn175) was expressed with an initial Met at the N-terminal.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti