分子别名(Synonym):LT,TNFB,TNFSF1
种属(Species):Human
表达系统(Expressed Host):E. coli
产品状态(Buffer):Lyophilized from sterile 50mM Tris, pH 8.0
分子量(Molecule Mass):The recombinant human TNFβ comprises 172 amino acids and predicts a molecular mass of 18.8 kDa as estimated in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 0.01-0.1 ng/mL.
表达区间(Protein Construction):A DNA sequence encoding the mature form of human TNFβ (P01374) (Leu 35-Leu 205) was expressed and purified, with an initial Met at the N-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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