分子别名（Synonym）:OMI,PARK13,PRSS25

种属（Species）：Human

表达系统（Expressed Host）：E. coli

产品状态（Buffer）：Lyophilized from sterile 50mM Tris, 0.3M NaCl, 1mM DTT, 20% Glycerol, pH 7.8

分子量（Molecule Mass）：The recombinant human HTRA2 consisting of 336 amino acids and has a calculated molecular mass of 36.5 kDa as estimated in SDS-PAGE under reducing conditions.

内毒素（Endotoxin）：＜1.0 EU per μg by the LAL method.

生物活性(Activity)：Protease activity demonstrated by HtrA2 cleavage of bovine β-casein (Sigma, Catalog # C-6905). Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45℃ in 50 mM Tris, pH 8.0, which results in >95% cleavage of β-casein, as revealed by SDS-PAGE.

表达区间（Protein Construction）：A DNA sequence encoding the mature form of human HTRA2 (O43464-1) (Ala 134-Glu 458) was expressed, with a polyhistide tag at the C-terminus.

存储条件（Storage）： For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.

运输条件： In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.