分子别名(Synonym):TFGA,TGFa
种属(Species):Human
表达系统(Expressed Host):E. coli
产品状态(Buffer):Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0.
分子量(Molecule Mass):The recombinant human TGFa consists of 50 amino acids and has a predicted molecular mass of 5.6 kDa. The apparent molecular mass of it is approximately 13 kDa in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):1. Measured by its binding ability in a functional ELISA. Immobilized human TGFa (cat:11252-HNAE) at 10 μg/ml (100 μl/well) can bind human EGFR-Fc (Cat:H1225-202H). The EC50 of human EGFR-Fc (Cat:H1225-202H) is 20-60 ng/ml.
表达区间(Protein Construction):A DNA sequence encoding the human TGFa (P01135-1) (Val40-Ala89) was expressed and purified.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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