分子别名(Synonym):1110037I22Rik,ALK3,AU045487,Bmpr,BMPR-IA
种属(Species):Mouse
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):The recombinant mouse ALK3/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 376 amino acids and has a predicted molecular mass of 42 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm ALK3/Fc monomer is approximately 60 kDa due to glycosylation.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 0.1-0.3 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.
表达区间(Protein Construction):A DNA sequence encoding the extracellular domain (Ser 2-Arg 152) of mouse ALK3 (NP_033888.2) precursor was fused with the Fc region of human IgG1 at the C-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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