纯度

> 95 % as determined by SDS-PAGE.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

Measured its binding ability with S1-RBD by ELISA.
Immobilized 2019-nCoV Spike Protein (RBD, mFc Tag)( Cat No.:nCoV-S) at 2μg/mL (100μL/well) can bind human ACE2 protein (His tag),the EC₅₀ of ACE2 is 20-90ng/mL

蛋白构建

A DNA sequence encoding the human ACE2 (NP_068576.1) (Met1-Ser740) was expressed with a 6×histidine tag at the C-terminus in HEK293 Cells.

表达宿主

HEK293 Cells

种属

Human

蛋白序列（预测）：

        10         20         30         40         50         60 
 QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS 
 
         70         80         90        100        110        120 
 TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN 
 
        130        140        150        160        170        180 
 PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE 
 
        190        200        210        220        230        240 
 DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS 
 
        250        260        270        280        290        300 
 PIGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS 
 
        310        320        330        340        350        360 
 VGLPNMTQGF WENSMLTDPG NVQKAVCHPT AWDLGKGDFR ILMCTKVTMD DFLTAHHEMG 
 
        370        380        390        400        410        420 
 HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN 
 
        430        440        450        460        470        480 
 FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY 
 
        490        500        510        520        530        540 
 CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLFNM 
 
        550        560        570        580        590        600 
 LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPYADQS 
 
        610        620        630        640        650        660 
 IKVRISLKSA LGDKAYEWND NEMYLFRSSV AYAMRQYFLK VKNQMILFGE EDVRVANLKP 
 
        670        680        690        700        710        720 
 RISFNFFVTA PKNVSDIIPR TEVEKAIRMS RSRINDAFRL NDNSLEFLGI QPTLGPPNQP 
 
 
 PVSHHHHHH

分子量

The recombinant human ACE2 consists of 729 amino acids and predicts a molecular mass of 84.4 kDa.

缓冲液

Sterile PBS, pH 7.4. 

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to 12 months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.