Catalog number: SAB90103H

Backgroud:

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG2a but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.

Physical State: Liquid                   

Applications: ELISA,WB

Suggested Dilution Range:

1:2000-1:40,000 for western blotting with ECL substrates;

1:1000-1:20,000 for ELISA and Western blotting with chromogenic substrates

Conjugation: Purified from horseradish root

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Storage Buffer: 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6

Storage: Store at -20°C. Avoid repeated freeze / thaw cycles.

For  Research  Use  Only !