分子别名(Synonym):GHR
种属(Species):Rat
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):The recombinant rat GHR comprises 258 amino acids and predicts a molecular mass of 29.6 kDa. The apparent molecular mass of the ratGHR is approximately 35-45 kDa in SDS-PAGE under reducing conditions due to glycosylation.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by its ability to inhibit proliferation of INS-1 cells induced by human growth hormone. The ED50 for this effect is 0.5-2μg/mL in the presence of 50 ng/mL human growth hormone.
表达区间(Protein Construction):A DNA sequence encoding the rat GHR (P16310-1) extracellular domain (Ser 2-Arg 265) was expressed, fused with a polyhistidine tag at the C-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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