分子别名（Synonym）:Il-12a,Il-12b,IL-12p35,Il-12p40,Il12p40,Ll12a,p35,p40

种属（Species）：Mouse

表达系统（Expressed Host）：HEK293 Cells

产品状态（Buffer）：Lyophilized from sterile PBS, pH 7.4

分子量（Molecule Mass）：The recombinant mouse IL12 heterodimer of IL12A/IL12B, IL12A is Fc chimera, comprises 758 (434 +324) amino acids and has a calculated molecular mass of 85.9 (48.7 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 45 kDa respectively in SDS-PAGE under reducing conditions.

内毒素（Endotoxin）：＜1.0 EU per μg by the LAL method.

生物活性(Activity)：1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL12RB2-His at 10 μg/ml (100 μl/well) can bind Mouse IL12A Cat: IL12B Heterodimer Protein (Cat:8H). The EC50 of Mouse IL12A Cat: IL12B Heterodimer Protein (Cat:8H) is 50.2-117.2 ng/ml.

表达区间（Protein Construction）：A DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Ser 2-Ala 215) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Ser 2-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

存储条件（Storage）： For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.

运输条件： In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.