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Mouse IL-12 (IL12A & IL12B Heterodimer) Protein


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产品品牌:Frdbio 产品货号:FrdMC1246-802H
保存温度: <- 20℃ 有效日期:12个月
英文名称:Mouse IL-12 (IL12A & IL12B Heterodimer) Protein
规      格: 20μg
价      格:优惠价 ¥1850.7
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分子别名(Synonym):Il-12a,Il-12b,IL-12p35,Il-12p40,Il12p40,Ll12a,p35,p40

种属(Species):Mouse

表达系统(Expressed Host):HEK293 Cells

产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4

分子量(Molecule Mass):The recombinant mouse IL12 heterodimer of IL12A/IL12B, comprises 528 (204 + 324) amino acids and has a calculated molecular mass of 60.3 (23.1 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 28 & 47 kDa respectively in SDS-PAGE under reducing conditions.

内毒素(Endotoxin):<1.0 EU per μg by the LAL method.

生物活性(Activity):1. Measured by its ability to bind biotinylated recombinant mouse IL12RB2 in a functional ELISA.

表达区间(Protein Construction):A DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Ser 2-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Ser 2-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.

运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

使用及储存:

Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti56366,595956367,595956364,36487503,36487497,36487498,36487509,36487512,19200078,19200034,19200035,19200064,19200065,19200076,36274759,36274767,36274757,36274761,595940420,34968589,36274763,36517339,23979205,23979210,23979201,38040275,595964448,595964483,595964482,595952657,595964449,38040273,38040272,5959

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