Product name: β-actin Mouse mAb

Catalog No.: RAB0100

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Molecular Weight:43 KD

Store at -20°C. Do not aliquot the antibody.

Recommended dilutions:WB: 1:3,000    IHC: 1:200    IF: 1:200

Optimal dilutions should be determined by the end user.

Specificity: The β-actin mouse monoclonal antibody can detect endogenous β-actin proteins.

Background: β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.

Note: This product is for research use only,not for diagnostic or therapeutic use.