分子别名(Synonym):IL23A
种属(Species):Rat
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4.
分子量(Molecule Mass):The recombinant rat IL23A & the rat IL12B comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.2 (21+37.2) KDa. The apparent molecular mass of the rat IL23A & the rat IL12B heterodimer is approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 2-15 ng/mL.
表达区间(Protein Construction):A DNA sequence encoding the rat IL23A (NP_569094.1) (Ser2-Ala196) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rat IL12B (EDM04146.1) (Ser2-Ser335) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the rat IL23A & the rat IL12B heterodimer was purified.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti56366,595956367,595956364,36487503,36487497,36487498,36487509,36487512,19200078,19200034,19200035,19200064,19200065,19200076,36274759,36274767,36274757,36274761,595940420,34968589,36274763,36517339,23979205,23979210,23979201,38040275,595964448,595964483,595964482,595952657,595964449,38040273,38040272,5959