分子别名(Synonym):IL-27,IL-27p28,Il30,p28
种属(Species):Mouse
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):The recombinant mouse IL27 comprises 442 amino acids and has a predicted molecular mass of 49.5 kDa. The apparent molecular mass of the protein is approximately 60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc (Cat:H1713-202H), The EC50 of human IL27RA-Fc (Cat:H1713-202H) is 0.26-0.62 μg/ml.
表达区间(Protein Construction):A DNA sequence encoding the mouse IL27 complex composed of EBI3 (O35228) (Ser2-Pro228) and IL27A (Q8K3I6) (Phe29-Ser234) linked by an polypeptide linker was fused with the polyhistidine tag at the C-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti56366,595956367,595956364,36487503,36487497,36487498,36487509,36487512,19200078,19200034,19200035,19200064,19200065,19200076,36274759,36274767,36274757,36274761,595940420,34968589,36274763,36517339,23979205,23979210,23979201,38040275,595964448,595964483,595964482,595952657,595964449,38040273,38040272,5959