分子别名(Synonym):S
种属(Species):MERS-CoV
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol.
分子量(Molecule Mass):The recombinant spike protein S1 (Human betacoronavirus 2c EMC/2012) comprises 719 amino acids and has a predicted molecular mass of 79.9 kDa. It migrates as an approximately 94 kDa band in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):1. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725)(Cat:M1293-802H) at 10 μg/ml (100 μl/well) can bind biotinylated human DPP4 (Cat:H1912-NCH).
表达区间(Protein Construction):A DNA sequence encoding the spike protein S1 (Human betacoronavirus 2c EMC/2012)( AFS88936.1)(Ser2-Glu725) was fused with a polyhistidine tag at the C-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti56366,595956367,595956364,36487503,36487497,36487498,36487509,36487512,19200078,19200034,19200035,19200064,19200065,19200076,36274759,36274767,36274757,36274761,595940420,34968589,36274763,36517339,23979205,23979210,23979201,38040275,595964448,595964483,595964482,595952657,595964449,38040273,38040272,5959