分子别名(Synonym):AA409063,CD142,Cf-3,Cf3,TF
种属(Species):Mouse
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):The secreted recombinant mouse F3 consists of 234 amino acids and has a calculated molecular mass of 26.8 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 40-45 kDa in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC (RCat:D Systems, Catalog# ES011).
表达区间(Protein Construction):A DNA sequence encoding the extracellular domain of mouse F3 (NP_034301.3) (Ser 2-Glu 251) was expressed, with a C-terminal polyhistidine tag.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti