分子别名(Synonym):AA960649,ARC-1,E-cad,Ecad,L-CAM,Um,UVO
种属(Species):Mouse
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):The secreted recombinant mouse CDH1 (pro form) comprises 697 amino acids and has a calculated molecular mass of 78 kDa. As a result of different glycosylation, the apparent molecular mass of rm CDH1 is approximately 80-100 kDa in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When 5 x 10E4 cells/well are added to Recombinant Mouse Cadherin-1 coated plates (0.8 μg/mL with 100 μL/well), approximately >50% will adhere after 1 hour at 37℃.
表达区间(Protein Construction):A DNA sequence encoding the extracellular domain of mouse CDH1 (P09803) (Ser 2-Val 709) was expressed, with a C-terminal polyhistidine tag.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti