分子别名(Synonym):AI853494,BEN,CD166,DM-GRASP,MuSC,SC1
种属(Species):Mouse
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):The recombinant mouse ALCAM/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 748 amino acids and has a predicted molecular mass of 84.2 kDa. Due to glycosylation, in SDS-PAGE under reducing conditions, rmALCAM/Fc migrates with approximately molecular mass of 105-110 kDa & 210 kDa corresponding to the monomer and homodimer.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Immobilized mouse CD6-His (Cat:M1935-802H) at 10 μg/ml (100 μl/well) can bind mouse ALCAM-Fc2h, The EC50 of mouse ALCAM-Fc2h is 0.12-0.28 μg/ml.
表达区间(Protein Construction):A DNA sequence encoding the extracellular domain (Ser 2-Lys 527) of mouse ALCAM (NP_033785.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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