分子别名(Synonym):ASP2,BACE,HSPC104
种属(Species):Human
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0
分子量(Molecule Mass):The recombinant human BACE1 consists of 442 amino acids and has a calculated molecular mass of 49 kDa. As a result of glycosylation, the rhBACE1 migrates as an approximately 65 kDa protein in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2 (Catalog# ES004, RCat:D Systems). Cleavage of ES004 can be measured using excitation and emission wavelength of 320 and 405 nm, respectively.
表达区间(Protein Construction):The mature form of human BACE1 (NP_036236.1) extracellular domain (Ser2-Thr 457) with a quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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