关键词:抗体制备抗体标记重组蛋白表达

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Human IGFBP3 / IBP3 Protein (His Tag)


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产品品牌:Frdbio 产品货号:FrdH1654-801H
保存温度: <- 20℃ 有效日期:12个月
英文名称:Human IGFBP3 / IBP3 Protein (His Tag)
规      格: 500μg
价      格:优惠价 ¥11213.65
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分子别名(Synonym):BP-53,IBP3,IGFBP3

种属(Species):Human

表达系统(Expressed Host):HEK293 Cells

产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4

分子量(Molecule Mass):The secreted recombinant human IGFBP3 comprises 280 amino acids with a predicted molecular mass of 31 kDa. As a result of glycosylation, the apparent molecular mass of rhIGFBP3 is approximately 40-45 kDa in SDS-PAGE under reducing conditions.

内毒素(Endotoxin):<1.0 EU per μg by the LAL method.

生物活性(Activity):1. Measured by its binding ability in a functional ELISA. Immobilized human IGF2 (Cat:13) at 10 μg/mL (100 μl/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 18 ng/mL.

表达区间(Protein Construction):A DNA sequence encoding the mature form of human IGFBP3 (NP_000589.2) (Gly28-Lys 291) was fused with a polyhistidine tag at the N-terminus.

存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.

运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

使用及储存:

Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantiI

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