分子别名(Synonym):C17orf33,C17orf33OS,CSF3OS,G-CSF,GCSF
种属(Species):Human
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
分子量(Molecule Mass):The recombinant mature human GCSFb/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 412 amino acids and has a calculated molecular mass of 45.4 kDa. As a result of glycosylation, the rh GCSFb/Fc monomer migrates as an approximately 48 kDa protein in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60. The ED50 for this effect is typically 0.2-0.8 ng/ml.
表达区间(Protein Construction):A DNA sequence encoding the mature form of human GCSF isoform b (NP_757373.1) (Ala 30-Pro 204) was fused with the Fc region of human IgG1 at the N-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti