分子别名(Synonym):CAIX,Carbonic Anhydrase IX,MN
种属(Species):Human
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
分子量(Molecule Mass):The recombinant human CA9/Fc is a disulfide-linked homodimer. The reduced monomer consists of 615 amino acids and predicts a molecular mass of 67.7 kDa. As a result of glycosylation, the apparent molecular mass of rh CA9/Fc monomer is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by its esterase activity. The specific activity is >50 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 1 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
表达区间(Protein Construction):A DNA sequence encoding the human carbonic anhydrase IX (CA9) precursor (NP_001207.2) (Ser 2-Asp 414) was fused with the Fc region of human IgG1 at the C-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti