分子别名(Synonym):CD100,coll-4,Semacl2,Semaj,Semcl2
种属(Species):Mouse
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):The secreted recombinant mouse SEMA4D comprises 721 amino acids and has a calculated molecular mass of 80.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by its ability to bind human SEMA4A in a functional ELISA.
表达区间(Protein Construction):A DNA sequence encoding the mouse SEMA4D (NP_038688.2) extracellular domain (Ser 2-Arg 733) was expressed, with a C-terminal polyhistidine tag.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti