关键词:抗体制备抗体标记重组蛋白表达

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Human TNFR2 / CD120b / TNFRSF1B Protein (His Tag)


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产品品牌:Frdbio 产品货号:FrdH1641-802H
保存温度: <- 20℃ 有效日期:12个月
英文名称:Human TNFR2 / CD120b / TNFRSF1B Protein (His Tag)
规      格: 50μg
价      格:优惠价 ¥2159.15
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分子别名(Synonym):CD120b,p75,p75TNFR,TBPII,TNF-R-II,TNF-R75,TNFBR,TNFR1B,TNFR2,TNFR80

种属(Species):Human

表达系统(Expressed Host):HEK293 Cells

产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4

分子量(Molecule Mass):The recombinant human TNFRII comprises 246 amino acids and has a calculated molecular mass of 26.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFRII is approximately 42-45 kDa due to glycosylation.

内毒素(Endotoxin):<1.0 EU per μg by the LAL method.

生物活性(Activity):Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-0.8 μg/ml in the presence of 0.25 ng/mL recombinant human TNF-α.

表达区间(Protein Construction):A DNA sequence encoding the extracellular domain (Ser 2-Asp 257) of human TNFRII (NP_001057.1) was expressed, fused with a C-terminal polyhistidine tag.

存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.

运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

使用及储存:

Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti

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