分子别名(Synonym):CD120b,p75,p75TNFR,TBPII,TNF-R-II,TNF-R75,TNFBR,TNFR1B,TNFR2,TNFR80
种属(Species):Human
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):The recombinant human TNFRII comprises 246 amino acids and has a calculated molecular mass of 26.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFRII is approximately 42-45 kDa due to glycosylation.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-0.8 μg/ml in the presence of 0.25 ng/mL recombinant human TNF-α.
表达区间(Protein Construction):A DNA sequence encoding the extracellular domain (Ser 2-Asp 257) of human TNFRII (NP_001057.1) was expressed, fused with a C-terminal polyhistidine tag.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti