分子别名(Synonym):CD31,CD31/EndoCAM,endoCAM,GPIIA',PECA1,PECAM-1
种属(Species):Human
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
分子量(Molecule Mass):The recombinant human CD31/Fc is a disulfide-linked homodimer.The reduced monomer consists of 812 amino acids and predicts a molecular mass of 91.2 kDa. As a result of glycosylation, the rhCD31/Fc monomer migrates as approximately 130-140 kDa band in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 × 10E4 cells/well are added to sCD31-Fc coated plates (1.25μg/mL, 100μL/well) in the presence of 20ng/mL PMA, approximately 20-45% will adhere after 30 minutes at 37℃.
表达区间(Protein Construction):A DNA sequence encoding the extracellular domain (Ser 2-Lys 601) of human CD31 (EAW94208.1) was expressed with the fused Fc region of human IgG1 at the C-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti 5516}