分子别名(Synonym):CD32,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2,FCGR2A1,IGFR2
种属(Species):Human
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):The recombinant human CD32a consists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.6 kDa. It migrates as an approximately 32 kDa protein due to glycosylation by SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Immobilized human CD32a-AVI-His (R131) (Cat:H1598-927H) at 10 μg/ml (100 μl/well) can bind biotinylated Human IgG1, The EC50 of biotinylated Human IgG1 is 0.15-0.35 μg/ml.
表达区间(Protein Construction):A DNA sequence encoding the extracellular domain (Ser 2-Met 210) of human CD32a (AAA35827.1) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus. It is identical to FCGR2A131H/R in the reference.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti 5516} ࡹ