分子别名(Synonym):CD4
种属(Species):Ferret
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):The recombinant Ferret CD4 consists of 386 amino acids and has a calculated molecular mass of 42.7 kDa. The apparent molecular mass of the recombinant protein is approximately 52 kDa in SDS-PAGE under reducing conditions due to glycosylation.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When 5 × 10E4 cells/well are added to CD4-coated plates (1.25μg/mL and 100μL/well),approximately >50% will adhere specifically after 30 minutes at 37℃.
表达区间(Protein Construction):A DNA sequence encoding the extracellular domain of Ferret (Mustela putorius furo) CD4 (ABS50090.1) (Ser 2-Leu 401) was expressed, with a polyhistidine tag at the C-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti 5516}