分子别名(Synonym):CD50,CDW50,ICAM-3,ICAM-R
种属(Species):Human
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):The recombinant human ICAM3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 703 amino acids and predicts a molecular mass of 77.2 kDa. By SDS-PAGE, the apparent molecular mass of rh ICAM3/Fc is approximately 125-135 kDa due to the glycosylation.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM3-coated plates (12.5 μg/ml, 100 μl/well), approximately 45%-60% will adhere specifically
表达区间(Protein Construction):The extracellular domain (Ser 2-His 485) of human ICAM3 (NP_002153.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti 5516}