分子别名(Synonym):CLMF,IL-12A,NFSK,NKSF1,P35
种属(Species):Human
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4.
分子量(Molecule Mass):The recombinant human IL12A&IL27B comprises 905 (447+458) amino acids and has a calculated molecular mass of 102 (50.7+ 51.3) KDa. The apparent molecular mass of the recombinant human IL12A&IL27B is approximately 60 & 63 KDa respectively in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):
表达区间(Protein Construction):A DNA sequence encoding the p35 subunit of human IL12, termed as IL12A (NP_000873.2) (Ser2-Ser219) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human IL27B, (NP_005746.2) (Ser2-Lys229) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti ↓_xFFFF_ Ɛ 蘀䀀܀Ebrima ᨓ↓_xFFFF_ Ɛ 蘀䀀܀宋体 ⨠ቻ↓_xFFFF_ Ɛ 蘀䀀܀Leelawadee UI 怈᧾↓_xFFFF_ Ɛ 蘀䀀܀Nirmala UI 쀘ᩪ↓_xFFFF_ ↓_xFFFF_ Ɛ 蘀䀀܀Ebrima ᨓ↓_xFFFF_ Ɛ 蘀䀀܀宋体 ⨠ቻԀ쭿¹ᨉ펠̧