分子别名(Synonym):CMAP
种属(Species):Human
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):Cystatin F exists as a disulfide-linked homodimer. The human cystatin F monomer consists of 137 amino acids and and has a predicted molecular mass of 16.1 kDa. As a result of glycosylation, the recombinant Cystatin F migrates as an approximately 44 kDa band in SDS-PAGE under non-reduced conditions corresponding to the dimmer.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (RCat:D Systems, Catalog # ES008). The IC50 value is <6 nM.
表达区间(Protein Construction):A DNA sequence encoding the human Cystatin F precursor (NP_003641.3) (Ser 2-His 145) was expressed,with a polyhistidine tag at the C-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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