分子别名（Synonym）:D1S1733E,DAN,DAND1,NB,NO3

种属（Species）：Human

表达系统（Expressed Host）：HEK293 Cells

产品状态（Buffer）：Lyophilized from sterile PBS, pH 7.4

分子量（Molecule Mass）：The recombinant human DAN consists of 176 amino acids and has a calculated molecular mass of 19.3 kDa. As a result of glycosylation, rh DAN migrates as an approximately 27 kDa protein in SDS-PAGE under reducing conditions.

内毒素（Endotoxin）：＜1.0 EU per μg by the LAL method.

生物活性(Activity)：Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 5-20 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.

表达区间（Protein Construction）：A DNA sequence encoding the human DAN precursor (NP_005371.1) (Ser 2-Asp 180) was expressed with a C-terminal polyhistidine tag.

存储条件（Storage）： For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.

运输条件： In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.