分子别名(Synonym):EDM1,EPD1,MED,PSACH,THBS5
种属(Species):Human
表达系统(Expressed Host):HEK293 Cells
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4
分子量(Molecule Mass):The mature form of human COMP consists of 748 amino acids after removal of the signal peptide and predicts a molecular mass of 82.4 kDa. As a result of glycosylation, the apparent molecular mass of rhCOMP is approximately 120-130 kDa in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. When cells are added to coated plates (5 μg/ml, 100 μl/well), approximately 40% will adhere specifically after 60 minutes at 37 ℃.
表达区间(Protein Construction):A DNA sequence encoding the human COMP (NP_000086.2) precusor (Ser 2-Ala 757) was expressed with a C-terminal polyhistidine tag.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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