分子别名(Synonym):IL-27,IL-27A,IL27A,IL27p28,IL30,p28
种属(Species):Human
表达系统(Expressed Host):CHO Stable Cells
产品状态(Buffer):Lyophilized from sterile PBS, 10% glycerol
分子量(Molecule Mass):The recombinant human IL27 complex (IL27B & IL27A) consists of 450 amino acids after removal of the signal peptide and has a calculated molecular mass of 56 KDa as estimated in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):1. Measured by its binding ability in a functional ELISA. Immobilized human IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc (Cat:H1713-202H), The EC50 of human IL27RA-Fc (Cat:H1713-202H) is 0.2-0.46 μg/ml.
表达区间(Protein Construction):A DNA sequence encoding the IL27 complex composed of IL27B subunit (NP_005746.2) (Ser 2-Lys 229) and the N-terminally truncated IL27p28 (IL27A) subunit (NP_663634.2) (Phe 29-Pro 243) linked by an polypeptide linker was fused with a polyhistidine tag at the C-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti ࿒ ࿒ 5516} ā