分子别名（Synonym）:R-spondin,Rspondin

种属（Species）：Mouse

表达系统（Expressed Host）：CHO Stable Cells

产品状态（Buffer）：Lyophilized from sterile PBS, pH 7.4

分子量（Molecule Mass）：The secreted recombinant mouse RSPO1 comprises 256 amino acids with a predicted molecular mass of 28.5 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions.

内毒素（Endotoxin）：＜1.0 EU per μg by the LAL method.

生物活性(Activity)：Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 50-200 ng/ml in the presence of 50 ng/ml recombinant mouse Wnt3a.

表达区间（Protein Construction）：A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Ser 2-Gln 265) was fused with a polyhistidine tag at the C-terminus.

存储条件（Storage）： For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.

运输条件： In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.