分子别名(Synonym):AI429687
种属(Species):Mouse
表达系统(Expressed Host):E. coli
产品状态(Buffer):Lyophilized from sterile PBS, pH 7.4.
分子量(Molecule Mass):The recombinant mouse CNTF consists of 215 amino acids and predicts a molecular mass of 24.7 KDa. It migrates as an approximately 25 KDa band in SDS-PAGE under reducing conditions.
内毒素(Endotoxin):<1.0 EU per μg by the LAL method.
生物活性(Activity):1. Measured by its binding ability in a functional ELISA. Immobilized mouse CNTF (cat:51181-M07E) at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His (Cat:R1243-802B). The EC50 of biotinylated rat CNTFR-His (Cat:R1243-802B) is 5-12 ng/ml.
表达区间(Protein Construction):A DNA sequence encoding the mouse CNTF (NP_740756.1) (Ala2-Ser298) was expressed with a polyhistidine tag at the N-terminus.
存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.
运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quanti 5516}